Establishment and optimization of high efficiency embryogenic callus induction system in Dendrobium candidum
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چکیده
منابع مشابه
Therapeutic Effect of Dendrobium candidum on Lupus Nephritis in Mice
CONTEXT Dendrobium candidum (D. candimum) widely is a functional drug. The curative effect of D. candidum on lupus nephritis has been studied in vivo. MATERIALS AND METHOD The DBA/2 and B6D2F1 mice were used for this in vivo experiment. The 50% effective dose (ED50) was used to check the effective concentration for this study. Then the SCr, BUN, TC, TG, IL-6, IL-12, TNF-α, and IFN-γ levels we...
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alstroemeria (alstroemeriaceae family) is an ornamental monocot mainly produced for its cut flowers. embryogenic calluses have been employed for gene transformation in alstroemeria. this research work was undertaken to study the embryogenic callus induction in alstroemeria cv. fuego. the vegetative explants (node, internode, leaf) along with various concentrations of auxins (picloram, naa, iaa,...
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Some physiological and biochemical changes were measured between embryogenic and non-embryogenic callus obtained from Cardiospermum halicacabum. Combination of auxin with cytokinin was more favourable for high amount of callus formation. 2,4-D played a key role in triggering somatic embryo formation. Embryogenic callus had more total carbohydrate and starch contents, total free amino acids, nuc...
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The development of somatic cells in to embryogenic cells occurs in several stages and ends in somatic embryo formation, though most of these biochemical and molecular changes have yet to be elucidated. Somatic embryogenesis coupled with genetic transformation could be a biotechnological tool to improve potential crop yields potential in sugarcane cultivars. The objective of this study was to ob...
متن کاملIn vitro Callus Induction and Rosmarinic Acid Quantification in Callus Culture of Satureja khuzistanica Jamzad (Lamiaceae)
In the present study, an efficient protocol has been developed for callus induction and production of RA in callus culture of Satureja khuzistanica for the first time. In vitro callus induction was achieved from young shoot tip explants cultured on MS and B5 media supplemented with different concentrations of IBA (0.1, 1.0, 2.0 and 5.0 mgl-1) solely or in combination with cytokinins BAP and KIN...
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ژورنال
عنوان ژورنال: African Journal of Plant Science
سال: 2016
ISSN: 1996-0824
DOI: 10.5897/ajps2015.1389